Experimental & Molecular Medicine
The long non-coding RNA-encoded microprotein MKKS3 drives colorectal cancer chemoresistance
Abstract Although oxaliplatin is widely used in the frontline treatment of colorectal cancer (CRC), CRC recurrence is commonly observed owing to oxaliplatin resistance. The roles of long non-coding RNAs-encoded microproteins in oxaliplatin chemoresistance remain inadequately explored. In this study, a CRISPR/Cas9 library was developed to target previously identified 314 microproteins translated from long non-coding RNAs, followed by functional screening. This strategy identified MKKS3, encoded by NR_072977, as a ke …