Evaluation of the efficiency of surface modification of nonwoven matrices with various polymer compositions using a cationic amphiphile: in vitro study

Vollständiger Abstract

Worum geht es in dieser Arbeit?

The potential for chemical modification of the surface of matrices composed of various natural and synthetic polymers using cationic amphiphiles remains insufficiently studied. Objective : to evaluate the biocompatibility and antimicrobial properties of polyurethaneand silk fibroin-based matrices modified with 1,4-diazabicyclo[2.2.2] octane (DABCO) attached to the surface via sorption mediated by hydrophobic or electrostatic interactions. Materials and methods . Surface modification of non-woven matrices based on an 8% polyurethane solution and a 5% regenerated silk fibroin solution was performed by sorption of DABCO through hydrophobic interactions (variant 0) or electrostatic interactions (variants 1 and 2). The minimum inhibitory concentration (MIC) of the cationic amphiphile was determined using serial dilution in 96-well plates against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Salmonella enterica ATCC 14028, Citrobacter freundii ATCC 8090, and Pseudomonas aeruginosa ATCC 9027. The amount of DABCO adsorbed on scaffold surfaces was determined by ultraviolet spectroscopy based on its residual concentration in the initial solutions. Surface structure, physicomechanical properties, antimicrobial activity, and biocompatibility of the scaffolds were assessed before and after modification. The obtained data were processed using GraphPad Prism 8. Results . The MIC of DABCO ranged from 2 to 8 μM. Modification variant 0 preserved scaffold surface structure and physicomechanical properties. Variants 1 and 2 improved scaffold surface hemocompatibility but made the scaffolds rigid and brittle. All modification variants demonstrated comparable antibacterial activity and biocompatibility. Conclusion . Surface modification of polyurethane and silk fibroin scaffolds with the cationic amphiphile DABCO via sorption mediated by hydrophobic or electrostatic interactions is suitable for imparting antibacterial properties to scaffolds.