P0016 Exploring DPP4 in intestinal inflammation: a cross-matrix approach using the TNBS-induced model of colitis

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Abstract Background Inflammatory Bowel Disease is an increasingly prevalent chronic intestinal disorder1. Biomarkers of disease are still an unmet need. Dipeptidyl peptidase 4 (DPP4), a membrane enzyme with immunoregulatory roles, can be shed into biological fluids2, but research as a potential biomarker of disease is scarce. This study evaluated DPP4 levels in the serum, colon and feces of rats with and without colitis. Methods This study was approved by institutional/national welfare bodies. Male Wistar Han rats (10–12 weeks) were randomly assigned to control (n = 10) or TNBS-induced colitis (n = 10) groups. Following 8h fasting with 5% sucrose ad libitum, TNBS rats received a rectal instillation of TNBS (18 mg/rat, 250 µL of 21% ethanol) under isoflurane anesthesia. Tramadol (20 mg/kg, PO) and paracetamol (500 mg/kg, PO) were administered daily for analgesia, while metoclopramide (1 mg/kg, PO) was given from day−1 to day2 to enhance intestinal motility. On day7, animals were euthanized, and serum, proximal and distal colon (PC, DC), intestinal content and cage fecal pellets (CFP) were collected. Colitis severity was assessed by macroscopic and microscopic scores. CFP from germ-free and DPP4 knockout (KO) mice and corresponding controls were also analysed. DPP4 was quantified by ELISA, and its localization was evaluated by immunohistochemistry (IHC). Statistical analyses were performed in GraphPad Prism and p